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Some isolates of the fungus Neurospora crassa possess a chromosomal factor that causes spore killing, leading to death of ascospores. It has been shown that these chromosomal factors are genetic elements called spore killers. For example, if a cross is performed between a parent with an Sk-S (sensitive) allele and a parent with an Sk-K (killer) allele, the cross will produce half viable offspring and half inviable offspring, where the inviable half has been killed by spore killing. This phenomenon can be explained by meiotic drive, wherein a selfish gene disrupts the randomness of sexual transmission, favoring its own success. In this study, I focus on a Neurospora Spore killer known as Sk-3. Sk-3 is thought to possess both a killer element and a resistance element. The resistance element is rsk, a gene that keeps ascospores alive and viable when in the presence of the killer element. However, the mechanism by which the killer element kills ascospores is unknown. A major obstacle to studying the killing mechanism is that the identity of the Sk-3 killer element itself has remained elusive. My goal is to help identify the Sk-3 killer element. Preliminary results by others have narrowed the search to the left arm of Chromosome III. These results have also shown that deletion of a 1.3 kb DNA interval, called v350, causes loss of spore killing. This suggests that a regulatory element, or a hidden gene, may overlap with the v350 interval. To help determine why v350 deletion correlates with loss of spore killing, I investigated a related DNA interval, called v384. My results suggest that v384, like v350, is required for spore killing.